Lipoprotein lipase (LpL) on the surface of cardiomyocytes increases lipid uptake and produces a cardiomyopathy
J. Clin. Invest. Hiroaki Yagyu, et al. 111:419 doi:10.1172/JCI16751 [Go to this article.]

Figure 3
LpL expression in plasma and hearts. (a) Postheparin plasma LpL activity. There was no difference in postheparin LpL activity between LpL1 and three lines of hLpL^GPI/LpL1 mice. LpL1, n = 6; line 346, n = 3; line 357, n = 6; line 358, n = 2. (b) Heart LpL activity. Hearts from control and three lines of male transgenic animals were homogenized and assayed for LpL activity in triplicate. Homogenates of hearts of hLpL^GPI/LpL1 mice (line 357, n = 3) had 3.8-fold more LpL activity than control LpL1 mice (n = 4). *P < 0.01. (c) Myocardial human LpL. Human LpL was differentiated from mouse LpL using an mAb against human LpL activity. All the additional LpL activity in hearts from hLpL^GPI/LpL1 mice (line 357, n = 3) was inhibited by the Ab, and no inhibition was found when the Ab was added to homogenates from control LpL1 hearts (n = 4). The graph shows the amount of activity inhibited by the Ab. Values are expressed as means ± SD. *P < 0.01. (d) Northern blot analysis of hLpL^GPI mouse tissue RNA. Ten micrograms of total heart RNA from male mice was subjected to Northern blot analysis. Probe is shown in Figure 1c. The hLpL^GPI mRNA was detected only in the hearts. H, heart; M, skeletal muscle; A, adipose; Lu, lung; Li, liver; K, kidney; S, spleen. (e) Lipoprotein profiles of LpL1 and hLpL^GPI/LpL1 mice. Cholesterol distribution for LpL1 mice is shown with open circles and hLpL^GPI/LpL1 mice with filled circles.