Massive hepatic apoptosis associated with TGF-β1 activation after Fas ligand treatment of IGF binding protein-1–deficient mice
J. Clin. Invest. Julia I. Leu, et al. 111:129 doi:10.1172/JCI16712 [Go to this article.]

Figure 4
Accumulation of fibronectin in the hepatocytes of IGFBP-1^–/– quiescent livers and breakdown of fibronectin in Fas-treated IGFBP-1^–/– livers. (a–f) Immunohistochemical staining of IGFBP-1^+/+ quiescent liver (a), IGFBP-1^–/– quiescent liver (b and c), IGFBP-1^–/– liver 7 hours after Fas treatment (d), IGFBP-1–pretreated IGFBP-1^–/– liver 7 hours after Fas treatment (e), and IGFBP-1^+/+ liver 7 hours after Fas treatment (f) sections stained using anti-fibronectin Ab. Scale bars: 50 μm except in c, which is 10 μm. (g) Western blot analysis comparing degradation of fibronectin in IGFBP-1^+/+, IGFBP-1^–/–, and IGFBP-1–pretreated IGFBP-1^–/– livers 7 hours after Fas challenge.