The antitumor effects of IFN-α are abrogated in a STAT1-deficient mouse
J. Clin. Invest. Gregory B. Lesinski, et al. 112:170 doi:10.1172/JCI16603 [
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Figure 6Absence of STAT1 in the host results in decreased signaling in response to IFN-α and decreased survival after tumor challenge. (
a) A DNA probe specific for activated murine STAT1 reacted with whole-cell lysates from IFN-α–treated splenocytes of C57BL/6 but not STAT1-deficient mice. Similar results were obtained in other tissues, including liver and kidney (data not shown).
STAT1–/– and C57BL/6 mice (
n = 10 mice per group) were injected intraperitoneally with 10
6 B16F1 melanoma cells. As compared with PBS-treated controls (filled circles), IFN-α treatment (2 × 10
4 U per day) (open triangles) prolonged the survival of C57BL/6 mice but not
STAT1–/– mice challenged with B16F1 melanoma cells (
b and
c) or JB/MS melanoma cells (
n = 6 mice per group) (
d and
e).
