Acceleration of type 1 diabetes mellitus in proinsulin 2–deficient NOD mice
J. Clin. Invest. Karine Thébault-Baumont, et al. 111:851 doi:10.1172/JCI16584 [
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Figure 1Genotyping of intercross mice for the gene (
a) and RT-PCR analysis of
proinsulin 2 gene expression (
b). (
a) A 675-bp PCR fragment corresponding to
proinsulin 2 (
Ins2) was observed in Ins2
+/+ and Ins2
+/– mice. All mice expressed a 187-bp PCR fragment corresponding to the
proinsulin 1 gene (
Ins1). (
b) Amplification of transcripts for proinsulin 2 using total thymic RNA from Ins2
+/+, Ins2
+/–, and Ins2
–/– mice. Total kidney RNA and islet RNA from Ins2
+/+ mice were used, respectively, as negative and positive controls. A 232-bp fragment was obtained by PCR in Ins2
+/+ and Ins2
+/– mice.
