Cancer-associated immunodeficiency and dendritic cell abnormalities mediated by the prostaglandin EP2 receptor
J. Clin. Invest. Li Yang, et al. 111:727 doi:10.1172/JCI16492 [Go to this article.]

Figure 6
Increased DC numbers draining lymph nodes of EP2-null mice. The number of (a) DCs, (b) CD4⁺, and (c) CD8⁺ T cells obtained from draining lymph nodes of normal or tumor-bearing EP2^–/– mice (white bars) is increased compared with wild-type control mice (black bars). For tumor-bearing mice, single-cell suspensions from inguinal lymph nodes from tumor-bearing EP2^–/– and wild-type mice were prepared 21 days after MC26 inoculation, at which time we observed the maximum tumor-growth difference. Cells were labeled with PE-conjugated anti–I-A^d_, CD11c Ab’s, anti-CD4, and anti-CD8 Ab’s. Cells were then washed and analyzed using a FACScan flow cytometer. The total number of positive cells per mouse is shown. Each data point is from three mice.