Cancer-associated immunodeficiency and dendritic cell abnormalities mediated by the prostaglandin EP2 receptor
J. Clin. Invest. Li Yang, et al. 111:727 doi:10.1172/JCI16492 [Go to this article.]

Figure 5
EP2^–/– DCs are refractory to mediated PGE₂ functional inhibition in vitro. PGE₂ induced inhibition of DC function signals through the EP2 receptor, shown by allogenic MLR. Day 9 DCs from EP2^–/– (white bars) and wild-type (black bars) mice on the BALB/c background, treated with PGE₂, were irradiated with 20 Gy, plated in triplicate, and incubated with T cells from wild-type mice with C57BL/6 background for 72 h. All cultures were pulsed with 1 μM [³H] thymidine 18 h before harvesting. Incorporation of [³H] thymidine was counted using a liquid scintillation counter and expressed as counts per minute. *EP2-null value was significantly different from wild-type value (P < 0.02). **EP2-null value is significantly different from EP2-null untreated control (P < 0.01).