Cancer-associated immunodeficiency and dendritic cell abnormalities mediated by the prostaglandin EP2 receptor
J. Clin. Invest. Li Yang, et al. 111:727 doi:10.1172/JCI16492 [Go to this article.]

Figure 2
Analysis of tumor angiogenesis in EP2^–/– and wild-type animals. (a) Staining of MC26 tumor sections with anti-CD31 Ab. Tumor samples were fixed with 4% paraformaldehyde, embedded in paraffin, and cut and stained using an anti-CD31 Ab. Blood vessels were visualized by the brown color of the CD31-labeled endothelial cells (×400). Slides were lightly counterstained with Mayer’s hematoxylin. (b) VEGF concentration of tumors from EP2^–/– and wild-type mice. Tumor tissue lysates were prepared by homogenization. Mouse VEGF was quantitated using ELISA. Data show VEGF production in pg per milligram of tumor proteins. Three mice each of EP2^–/– and wild type were used, with triplicates for each sample in the immunoassay.