Involvement of PTEN in airway hyperresponsiveness and inflammation in bronchial asthma
J. Clin. Invest. Yong-Geun Kwak, et al. 111:1083 doi:10.1172/JCI16440 [Go to this article.]

Figure 6
Localization of immunoreactive PTEN in BAL fluids of OVA-sensitized and -challenged mice. Sampling was performed 72 hours after the last challenge in BAL fluids from sensitized mice challenged with saline (a, e, and f), sensitized mice challenged with OVA (b), OVA-inhaled mice administered AdPTEN (c), and OVA-inhaled mice administered AdLacZ (d). Representative light microscopy shows PTEN-positive cells in the BAL fluids (a, b, c, d, and e); the brown color indicates PTEN-positive cells. (f) To examine the cell differentials in BAL cells prepared from the control mice, the slides used for the detection of PTEN (e) were destained with 70% ethyl alcohol. The smears of BAL cells were stained with Diff-Quik solution and viewed under a light microscope. The arrow indicates a macrophage; the arrowhead indicates a lymphocyte. Bars indicate 10 μm.