Chronic myelogenous leukemia shapes host immunity by selective deletion of high-avidity leukemia-specific T cells
J. Clin. Invest. Jeffrey J. Molldrem, et al. 111:639 doi:10.1172/JCI16398 [Go to this article.]

Figure 5
High-avidity PR1-specific CTLs undergo apoptosis 18 hours after stimulation with high-concentration PR1 peptide. PBMCs from a healthy donor 28 days after weekly restimulation with either 0.2 μM or 20 μM PR1-pulsed T2 cells established relatively high- and low-avidity PR1-CTL, respectively (far left panels). The resulting PR1-CTLs were washed and combined in a 1:1 ratio, based on the number of tetramer-positive cells, with T2 cells pulsed with either 0.2 μM or 20 μM PR1 peptide. After 16 to 18 hours, cells were stained with annexin V Ab, and live cells were analyzed based on PI staining. The percentage of CD8⁺ cells that are tetramer-positive is shown in the far left panels, and the percentage of tetramer-positive cells that stain with annexin V are shown in the remaining panels. (a) Annexin V expression increased on high-avidity PR1-CTLs exposed to high-concentration (20 μM) PR1, but not after exposure to low (0.2 μM) concentration PR1. Annexin V upregulation was blocked by pretreating peptide-pulsed T2 cells with anti–HLA-A2 (BB7.2) prior to coculture with PR1-CTL. (b) Annexin V was not upregulated 18 hours after coculture of low-avidity PR1-CTLs with either low-concentration (0.2 μM) or high concentration (20 μM) PR1 peptide.