Impaired Smad7-Smurf–mediated negative regulation of TGF-β signaling in scleroderma fibroblasts
J. Clin. Invest. Yoshihide Asano, et al. 113:253 doi:10.1172/JCI16269 [Go to this article.]

Figure 8
The inhibitory effect of Smad7 in normal and scleroderma fibroblasts. Cells were transfected with 2 μg of the –772COL1A2/CAT (a) or 3TP-lux construct (b), along with 0.1 μg of Smad7 or the corresponding empty construct. After a 24-hour incubation, the indicated reagent was added. Cells were harvested 48 hours after transfection. Values represent the promoter activity relative to that of untreated normal fibroblasts transfected with empty vector, which was set at 100 (a) or 10 AU (b). The mean and SEM results from five separate experiments are shown.