Impaired Smad7-Smurf–mediated negative regulation of TGF-β signaling in scleroderma fibroblasts
J. Clin. Invest. Yoshihide Asano, et al. 113:253 doi:10.1172/JCI16269 [Go to this article.]

Figure 6
Subcellular localization of Smad7 in normal and scleroderma fibroblasts. (a–i) The subcellular distribution of Smad7, TβRI, and TβRII was visualized by immunofluorescence. Smad7 was visualized with polyclonal goat anti-Smad7 Ab and FITC-conjugated donkey anti-goat IgG (green). TβRI and TβRII were detected with polyclonal rabbit anti-TβRI and TβRII Ab’s and TRITC-conjugated donkey anti-rabbit IgG (red), respectively. Colocalization of Smad7 and TGF-β receptors (overlay) appears as yellow. (j and k) Mv1Lu cells were transfected with the expression vector for Smad7. After a 48-hour incubation, Smad7 was visualized as described above (j). In some experiments, cells were treated with TGF-β1 (2 ng/ml) for the last 3 hours (k).