Impaired Smad7-Smurf–mediated negative regulation of TGF-β signaling in scleroderma fibroblasts
J. Clin. Invest. Yoshihide Asano, et al. 113:253 doi:10.1172/JCI16269 [
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Figure 3The phosphorylation levels of Smad2 in normal and scleroderma fibroblasts either treated or untreated [TGF-β1 (–)] with TGF-β1. Cell extracts were prepared from confluent quiescent fibroblasts either treated or untreated with 2 ng/ml of TGF-β1 for 1 hour, electrophoresed through a 10% polyacrylamide gel, transferred to a nitrocellulose membrane, and probed with anti–phospho-Smad2 Ab (pSmad2). After stripping, total levels of Smad2 were determined using anti-Smad2/Smad3 Ab. One representative of five independent experiments is shown.
