Early maternal hypothyroxinemia alters histogenesis and cerebral cortex cytoarchitecture of the progeny
J. Clin. Invest. Rosalía Lavado-Autric, et al. 111:1073 doi:10.1172/JCI16262 [Go to this article.]

Figure 1
Photomicrographs of coronal sections of primary somatosensory cortex (a–h) and hippocampus (i–l) showing BrdU-labeled cells in normal, LID-plus-KI, LID-1, and LID-2 pups at P40. BrdU labeling after E14–E16 (a–d) and E17–E19 (e–l) injections shows that both in the neocortex (a–h) and in the hippocampus (i–l), the radial distribution of BrdU-labeled cells is more widespread in LID-1 and LID-2 pups than in normal and LID-plus-KI pups. Note the increased number of heterotopic BrdU-labeled cells in the white matter of the somatosensory cortex (c and d) and in the strata oriens and alveus of CA1 of the progeny of LID-1 and LID-2 dams (k and l) as compared with the progeny of normal and LID-plus-KI dams (a and b and i and j, respectively). Horizontal lines indicate the borders between layers. Dashed lines indicate borders in g and h, since they are blurred in cresyl violet–stained adjacent sections. Rectangles in g and k show two examples of probes. In each probe, BrdU-labeled cells were plotted, and the relative frequency per layer was calculated. Borders between layers were established by superposing adjacent cresyl violet–stained sections. Strata pyramidale, radiatum, and moleculare are indicated. Magnification for a–d is 30×; 17× for e–h; and 36× for i–l. wm, white matter; or, stratum oriens; al, stratum alveus; py, stratum pyramidale; ra, stratum radiatum; mo, stratum moleculare; II–IV, layers II through IV; V–VI, layers V through VI.