Distinct progenitor populations in skeletal muscle are bone marrow derived and exhibit different cell fates during vascular regeneration
J. Clin. Invest. Susan M. Majka, et al. 111:71 doi:10.1172/JCI16157 [Go to this article.]

Figure 2
Analysis of vascular-specific gene expression in skeletal muscle-derived cells. RT-PCR analysis was performed on total RNA (50 ng/sample) isolated from distinct populations of skeletal muscle cells: (a) Percoll-fractionated skeletal muscle cells; (b) muscle SP population; and (c) muscle non-SP population. (d) Bone marrow-derived SP cells were directly compared to skeletal muscle-derived SP cells. GAPDH was used as a loading control. Analyses using each primer set were performed on three to five independent RNA isolates from each cell population; representative data are shown. The amplicon generated from each primer set was cloned and sequenced to verify that the primers amplify the intended gene. VE-cad, VE-cadherin; SMαA, SM-α-actin; m, marker; B, bone marrow SP; M, muscle SP.