Distinct progenitor populations in skeletal muscle are bone marrow derived and exhibit different cell fates during vascular regeneration
J. Clin. Invest. Susan M. Majka, et al. 111:71 doi:10.1172/JCI16157 [Go to this article.]

Figure 1
Isolation of murine skeletal muscle progenitors. (a) Skeletal muscle stem cells were isolated from 6- to 8-week-old C57Bl/6 or Rosa26 mice, fractionated on a Percoll gradient, stained with Hoechst 33342 dye, and subjected to FACS. SP and non-SP cells were clearly discernable based on Hoechst dye exclusion, and the distinct populations were isolated via FACS. (b) In this representative sample, the SP population was 0.21%, and the non-SP was 81.9% of the total viable cells. (c) Both SP and non-SP populations were cultured overnight, and their distinct morphologies were revealed upon microscopic examination of the cells in culture. The SP cells were found to be of uniform morphology and nonadherent. The non-SP population contained predominantly adherent, spindle-shaped cells.