Inhibition of endothelial lipase causes increased HDL cholesterol levels in vivo
J. Clin. Invest. Weijun Jin, et al. 111:357 doi:10.1172/JCI16146 [Go to this article.]

Figure 1
Characterization of the inhibitory effects of the anti-mEL antibody and its specificity. (a) Conditioned media from HEK293 cells transfected with the mEL cDNA were preincubated with increasing amounts of control IgG or anti-mEL IgG, and effects on triglyceride lipase and phospholipase activity were determined and compared with effects in untreated (PBS) media. The results are presented as the percentage of activity in medium preincubated with PBS alone and are the means ± SD of triplicate determinations. (b) Conditioned media from HEK293 cells transfected with mEL, mHL, and mLPL cDNAs were preincubated with PBS, control IgG, or anti-mEL IgG, and the effects on triglyceride lipase activity were determined. The results are presented as the percentage of activity in medium preincubated with PBS alone and are the means ± SD of triplicate determinations. (c) Homogenized liver lysates (15 μg protein) from wild-type, human apoA-I transgenic, and HL^–/– mice and conditioned media from mEL-, mHL-, and mLPL-transfected cells were separated by SDS-PAGE and immunoblotted with an anti-mEL antibody. Both forms of mEL were detected in the liver lysates of all three mouse models and were of slightly smaller size than mELs in conditioned media. The additional band detected in the human apoA-I transgenic and HL^–/– mouse liver lysates was also seen on immunoblotting of these same liver lysates using a control antibody (data not shown). No bands were seen in the mHL or mLPL conditioned media.