The α1A/C- and α1B-adrenergic receptors are required for physiological cardiac hypertrophy in the double-knockout mouse
J. Clin. Invest. Timothy D. O’Connell, et al. 111:1783 doi:10.1172/JCI16100 [
Go to this article.]
Figure 5Hypertrophic signaling in myocytes. Cultured myocytes from hearts of 10- to 11-week-old congenic WTand ABKO mice were treated for 15 minutes with PE (20 μM, plus 2 μM timolol); phorbol 12-myristate, 13-acetate (PMA, 100 nM); sorbitol (Sor, 1 M); endothelin (ET, 10 nM); insulin (Ins, 6 μM); or vehicle. Western blots were done for (
a) phospho-Erk1/2 (pErk) and total Erk1/2, (
b) phospho-p90RSK (pp90RSK), (
c) phospho-p70S6K (pp70S6K), and (
d) phospho-Akt (pAkt) and total Akt. In
a, the bar graphs summarize Erk1/2 data from six hearts in each group. In
b, a nonspecific (NS) band is indicated with anti–pp90RSK. In
d, a second pAkt antibody (S473) gave identical results (not shown). Total p90RSK (
b) and total p70S6K (
c) were the same in WT and ABKO mice (not shown). *
P < 0.05 vs. Veh.
