The α_1A/C- and α_1B-adrenergic receptors are required for physiological cardiac hypertrophy in the double-knockout mouse
J. Clin. Invest. Timothy D. O’Connell, et al. 111:1783 doi:10.1172/JCI16100 [Go to this article.]

Figure 3
Myocyte size in ABKO and WT mice. (a) Ventricular cross sections. LV coronal sections from 10- to 11-week-old male mice were stained with fluorescein-conjugated wheat germ agglutinin for sarcolemmal membranes and with Hoechst 33342 for nuclei. Nuclei between myocytes are in nonmyocytes. ×40. Scale bar, 100 μm. (b) Isolated myocytes. Myocytes were from hearts of 9- to 10-week-old male mice. ×20, phase contrast. (c) Myocyte volume. Myocyte volume by Coulter Multisizer is plotted versus myocyte number for representative hearts of each genotype and sex. (d) Myocyte cross-sectional area. Cross-sectional area in myocytes with a central nucleus, as in a, is plotted versus myocyte number (110–190 cells in each of two male hearts for each genotype). (e) Myocyte surface area. Surface areas of isolated myocytes as in b are plotted versus myocyte number (160–190 cells in each of three male hearts for each genotype). Group data are in Table 1.