Disruption of the CXCR4/CXCL12 chemotactic interaction during hematopoietic stem cell mobilization induced by GCSF or cyclophosphamide
J. Clin. Invest. Jean-Pierre Lévesque, et al. 111:187 doi:10.1172/JCI15994 [
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Figure 5Cleavage and inactivation of CXCL12 in mobilized BM is due to serine proteases. Aliquots of synthetic human CXCL12α were incubated overnight at 37°C in the presence of PBS (lane 1), BM extracellular fluids isolated on day 4 of GCSF mobilization (lanes 2–5) after preincubation in the absence of protease inhibitor (lane 2) or in the presence of human α1-antitrypsin (lane 3), PMSF (lane 4), or BB-94 (lane 5). In the top panel, samples (black bars) together with controls without exogenous CXCL12α were analyzed for chemotactic activity on Nalm-6 cells as described in Figure
4a. A representative experiment from two performed is shown. In the bottom panel, the same samples were analyzed by Western blot with a goat anti-human CXCL12 antibody. A representative experiment from two performed is shown.
