Disruption of the CXCR4/CXCL12 chemotactic interaction during hematopoietic stem cell mobilization induced by GCSF or cyclophosphamide
J. Clin. Invest. Jean-Pierre Lévesque, et al. 111:187 doi:10.1172/JCI15994 [Go to this article.]

Figure 3
CXCL12 concentration in the BM decreases when HPCs are mobilized in the PB. (a) BM extracellular fluids were extracted at the indicated time points from mice injected with either saline (open circles), CY alone (filled circles), GCSF alone (filled triangles), or CY in combination with GCSF (filled squares). CXCL12 concentrations were quantified by ELISA. (b) PB from mice injected with either CY alone (filled circles), GCSF alone (filled triangles), CY in combination with GCSF (filled squares), or saline (open circles) was taken at the indicated time points and plated in triplicate in clonogenic assays. The numbers of CFCs were determined after 14 days of incubation at 37°C. Data are means ± SD of three to six mice per group, with each sample analyzed in triplicate. Statistically significant differences with noninjected animals are indicated (*P < 0.05, **P < 0.01, as determined by Student’s t test).