Endothelial lipase provides an alternative pathway for FFA uptake in lipoprotein lipase–deficient mouse adipose tissue
J. Clin. Invest. Dagmar Kratky, et al. 115:161 doi:10.1172/JCI15972 [
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Figure 2Murine
EL mRNA expression in liver. Relative abundance of
EL mRNA in livers in the presence (
L2) or absence (
L0-MCK) of AT-LPL were compared with
EL expression in AT of
L0-MCK mice. Total RNA was isolated from fat pads of fed
L2 and
L0-MCK mice. mRNA concentrations were determined by fluorescent real-time PCR.
EL mRNA quantities were normalized to those of β-actin. Data are expressed as the mean values of 3 experiments performed in duplicate. Bars represent mean ± SD of the hybridization intensity signals. Inset: Representative Northern blot analysis of 10 μg total RNA per lane, hybridized with a murine-specific [
32P]-labeled EL cDNA probe. Lane 1,
L0-MCK AT; lane 2,
L2 liver; lane 3,
L0-MCK liver.
