Endothelial lipase provides an alternative pathway for FFA uptake in lipoprotein lipase–deficient mouse adipose tissue
J. Clin. Invest. Dagmar Kratky, et al. 115:161 doi:10.1172/JCI15972 [Go to this article.]

Figure 1
Murine LPL and EL mRNA expression in adipose tissue. Relative abundance of (A) LPL and (B) EL mRNA in AT of mice expressing (L2, L2-MCK) or lacking (L0-MCK, L0) LPL in AT. Total RNA was isolated from murine AT in the absence or presence of LPL and subjected to Northern blot analysis. Ten micrograms of total RNA were separated on a formaldehyde/agarose gel, blotted onto a nylon membrane, and hybridized with a murine-specific [³²P]-labeled cDNA probe. rRNA, ribosomal RNA. (C) EL mRNA concentrations in AT were confirmed by fluorescent real-time PCR (see Methods). EL mRNA quantities were normalized to those of β-actin. Data are expressed as the mean values of 3 experiments performed in duplicates relative to the expression of EL mRNA in control mice (L2). Bars represent mean ± SD of the hybridization intensity signals. *P < 0.05.