Differential subunit composition of the G protein–activated inward-rectifier potassium channel during cardiac development
J. Clin. Invest. Bernd K. Fleischmann, et al. 114:994 doi:10.1172/JCI15925 [Go to this article.]

Figure 2
The CCh effect is blocked by the I_K,ACh -selective blocker tertiapin. (A) Hyperpolarization and negative chronotropy induced by CCh (1 μM) are reversed by tertiapin (1 μM) in a late-stage atrial cardiomyocyte. (B) CCh-induced deceleration of spontaneous APs is reversed by tertiapin in late-stage but not in early-stage atrial cardiomyocytes. Data are mean ± SEM of 6 early- and 7 late-stage cells (P = 0.026 for control and P = 0.0003 for CCh, unpaired Student’s t test). (C) Tertiapin-induced depolarization of the membrane potential in CCh-treated early- and late-stage atrial cardiomyocytes (P = 0.0007, unpaired Student’s t test). Recovery was estimated by determining the maximal diastolic potential prior to application of CCh and in presence of CCh and tertiapin. Asterisks denote statistically significant difference.