Normal levels of anticoagulant heparan sulfate are not essential for normal hemostasis
J. Clin. Invest. Sassan HajMohammadi, et al. 111:989 doi:10.1172/JCI15809 [
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Figure 2Hs3st1 disruption reduces tissue HS
act level but does not alter fibrin accumulation. (
a) HS
act content of HS purified from tissues was measured as in vitro anti-Xa activity, where HS containing AT-binding sites catalyzes AT neutralization of factor Xa. Inset shows the activity of tissue HS to catalyze HCII neutralization of T. Activities were calibrated to the USP units of a heparin standard;
n = 3 littermates per group. (
b) Tissue fibrin accumulation was determined by first preparing urea-insoluble extracts, which selectively enriches for cross-linked fibrin. Fibrin was quantified by Western blots probed with a mAb specific to the cleaved β-chain of fibrin II. Each group contained five young adult mice and five middle-aged mice (∼10 or ∼24 weeks of age, respectively) with no significant effect of age on fibrin accumulation.
