The HIV protease inhibitor ritonavir blocks osteoclastogenesis and function by impairing RANKL-induced signaling
J. Clin. Invest. Michael W.-H. Wang, et al. 114:206 doi:10.1172/JCI15797 [
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Figure 8RANKL-induced recruitment of c-Src and TRAF6 to lipid raft component is inhibited by ritonavir treatment. Preosteoclasts generated after 3 days of culture with RANKL and M-CSF were starved for 3 hours, followed by pretreatment with either vehicle or ritonavir (1 hour). Cells were then stimulated with RANKL for 5 minutes, followed by lipid raft isolation. Note the continued recruitment of TRAF2 to lipid rafts with ritonavir treatment, indicating that the inhibition is specific to TRAF6 and c-Src.
