The HIV protease inhibitor ritonavir blocks osteoclastogenesis and function by impairing RANKL-induced signaling
J. Clin. Invest. Michael W.-H. Wang, et al. 114:206 doi:10.1172/JCI15797 [Go to this article.]

Figure 4
Ritonavir blocks PTH-induced osteoclast formation in vivo. (A) Osteoclast number was determined from TRAP-stained histologic sections of calvariae from mice stimulated with PTH or vehicle and intraperitoneally injected with ritonavir or vehicle. Ritonavir abrogates the osteoclast increase stimulated by PTH (n = 3 mice per group; P < 0.05). Cal. inj., calvarial injection; i.p. inj., intraperitoneal injection; Veh, vehicle. (B) Representative fields of TRAP-stained sections of calvariae show PTH injection fails to induce osteoclast formation in ritonavir-treated mice despite a robust PTH-dependent stromal cell response. Magnification, ×250.