Therapeutic efficacy of sonic hedgehog protein in experimental diabetic neuropathy
J. Clin. Invest. Nigel A. Calcutt, et al. 111:507 doi:10.1172/JCI15792 [
Go to this article.]
Figure 1Reduced
dhh mRNA in sciatic nerves of diabetic rats as shown by radioactive in situ hybridization. Hybridizations were performed on transverse sections of paraffin-embedded sciatic nerves from diabetic (
a and
b) and nondiabetic control (
c–
e) rats using antisense probes for
dhh (
a,
c, and
e) or
β-actin (
b and
d). In (
e), black arrows indicate signal above the nuclei of endoneurial Schwann cells, and gray arrows indicate nuclei of cells in the perineurial sheath that are unlabeled by the
dhh probe. Magnification, ×200 (
a–
d); ×400 (
e). In (
f), quantification of
dhh grain area relative to tissue-section area is shown in diabetic and nondiabetic control nerves. Data are given as means ± SEM;
n = 5 nerves per group. *
P < 0.05. D, diabetic; ND, nondiabetic; en, endoneurial; s, perineurial sheath.
