Altered cutaneous immune parameters in transgenic mice overexpressing viral IL-10 in the epidermis
J. Clin. Invest. Wanhong Ding, et al. 111:1923 doi:10.1172/JCI15722 [Go to this article.]

Figure 5
ECs and splenocytes from vIL-10Tg mice have decreased ability to stimulate allogeneic T cell proliferation. (a) Various numbers of ECs (stimulators; S) were prepared from vIL-10Tg mice and WT mice. These cells were cocultured with 2 × 10⁵ nylon wool-purified allogeneic (BALB/c) T cells (responders; R). Control ECs and T cells were cultured alone. T cell proliferation was measured by ³H-thymidine uptake. Data are expressed as the mean cpm of triplicate cultures ± SEM. (b) Various numbers of splenocytes (stimulators; S) were prepared from vIL-10Tg mice and WT mice and x-irradiated. These cells were cocultured with 2 × 10⁵ allogeneic (BALB/c) splenocytes (responders; R). Stimulators and responders were cultured alone as controls. T cell proliferation was measured by ³H-thymidine uptake. Data are expressed as the mean cpm of triplicate cultures ± SEM.