src homology 2 domain–containing tyrosine phosphatase SHP-1 controls the development of allergic airway inflammation
J. Clin. Invest. Tohru Kamata, et al. 111:109 doi:10.1172/JCI15719 [
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Figure 3Increased Th2 cell differentiation in
me/+ naive CD4 T cells. Naive CD4 T cells from wild-type and
me/+ mice were stimulated for 5 days with immobilized anti–TCR-β mAb (1 μg/ml) in the presence of 20 U/ml exogenous IL-2 and 3 U/ml IL-4 (Th2-skewed condition) or 1 U/ml IL-12 and anti–IL-4 mAb (Th1-skewed condition). The generation of Th1 and Th2 cells was assessed by cytoplasmic staining with IFN-γ and IL-4. The absolute numbers of cells harvested were similar in these cultures. The percentages of cells in each quadrant are shown. Four independent experiments were performed with similar results.
