src homology 2 domain–containing tyrosine phosphatase SHP-1 controls the development of allergic airway inflammation
J. Clin. Invest. Tohru Kamata, et al. 111:109 doi:10.1172/JCI15719 [Go to this article.]

Figure 2
TCR-induced MAPKK activation and IL-4–induced STAT6 phosphorylation in splenic CD4 T cells from me/+ mice. (a) Phosphorylation status of Erk1 and Erk2 in splenic CD4 T cells was assessed 5–60 minutes after co-cross-linking of TCR and CD4 molecules. The cells were lysed, and the lysates were subjected to immunoblotting with anti–phospho-Erk antibody. Densitometric measurements of the phosphorylated bands (p44 for Erk1 and p42 for Erk2) are shown under each band in arbitrary units. Erk1-P, phospho Erk-1. (b) IL-4–induced phosphorylation of STAT6. Splenic CD4 T cells from wild-type and me/+ mice were stimulated with IL-4 (100 U/ml) at 37°C for the indicated times. The cell lysates were subjected to immunoprecipitation with anti-STAT6 antiserum, and to immunoblotting with anti-phosphotyrosine mAb (upper panel) or anti-STAT6 antiserum (lower panel). Densitometric measurements of the phosphorylated STAT6 bands and STAT6 protein bands are shown under each band in arbitrary units. STAT6–P, phospho-STAT6.