src homology 2 domain–containing tyrosine phosphatase SHP-1 controls the development of allergic airway inflammation
J. Clin. Invest. Tohru Kamata, et al. 111:109 doi:10.1172/JCI15719 [
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Figure 1Phenotypically normal naive CD4 T cells developed in
me/+ mice. (
a) Representative CD4/CD8 profiles of splenocytes and thymocytes of wild-type (
+/+) and heterozygous (
me/+) mice with the
motheaten mutation. The percentages of cells in each quadrant are shown. The numbers of cells harvested (yield) were 1.41 × 10
8 splenocytes in wild-type, 1.52 × 10
8 splenocytes in
me/+, 1.72 × 10
8 thymocytes in wild-type, and 1.70 × 10
8 thymocytes in
me/+. (
b) Splenic CD4 T cells from wild-type and
me/+ mice were cultured with IL-4 (100 U/ml) for 24 hours, and the expression of common-γ and IL-4Rα chain was assessed. Background staining is shown as hatched areas. (
c) SHP-1 protein expression in splenic CD4 T cells from
me/+ and wild-type mice. Immunoprecipitation and immunoblotting with anti–SHP-1 mAb were performed. Ratios of the intensities of the SHP-1 bands are shown.
