The crucial role of Campylobacter jejuni genes in anti-ganglioside antibody induction in Guillain-Barré syndrome
J. Clin. Invest. Peggy C.R. Godschalk, et al. 114:1659 doi:10.1172/JCI15707 [Go to this article.]

Figure 4
SDS-PAGE analysis of LOS from GB11 WT and mutants. The genome strain NCTC 11168 was included as a control. Lane 1, WT GB11; lane 2, GB11 cst-II mutant; lane 3, GB11 orf10/orf11 mutant; lane 4, GB11 orf11 mutant; lane 5, NCTC 11168. (A) Silver staining of the LOS revealed faster-migrating LOS cores for the GB11 cst-II and orf10/orf11 mutants compared with the WT, indicating that these mutants have a truncated LOS. The orf11 mutant LOS showed migration patterns identical to those of WT LOS. (B) A Western blot incubated with GB11 patient serum showed a reduced reactivity for the cst-II and orf10/orf11 mutants but unchanged reactivity for the orf11 mutant when compared with the WTs. (C) For the cst-II and orf10/orf11 mutants, the reactivity with cholera toxin, a ligand for GM1-oligosaccharide structures, was almost completely lost. Reactivity with the orf11 mutant remained unchanged.