Impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis in noggin-overexpressing mice
J. Clin. Invest. Xue-Bin Wu, et al. 112:924 doi:10.1172/JCI15543 [
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Figure 7Phenotype of a 4-month-old noggin-overexpressing mouse. Expression of noggin mRNA detected by RT-PCR in liver (L), kidney (K), and bone (B), and by Western blotting in bone (
a) in WT and noggin transgenic (TG) littermates; GAPDH was used as a control. (
b) Radiology of the femur; (
c) bone mineral density of the femur, tibia, spine (L4–L6), and total body; and (
d) H&E staining of femur were performed to show differences between WT and noggin transgenic mice. Comparison of bone marrow cell cultures isolated from WT and noggin transgenic mice to evaluate ALP-positive CFU-F, CFU-OB (
e), and TRAP-positive osteoclast formation (
f). Results are expressed as mean ± SEM. *
P < 0.05, significantly different from WT mice by ANOVA.
