Impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis in noggin-overexpressing mice
J. Clin. Invest. Xue-Bin Wu, et al. 112:924 doi:10.1172/JCI15543 [
Go to this article.]
Figure 6Effects of BMP and ca-ALK-3 overexpression on osteoblast differentiation. (
a) Concentration-dependent stimulation of ALP activity in mature OB-6 osteoblasts by BMP-2 and BMP-4, expressed as absolute values. (
b) Concentration-dependent inhibition of ALP activity by noggin or PEG-noggin in the presence of a fixed dose of BMP-2 or BMP-4 (100 ng/ml), expressed as a percentage of the control value. (
c) Anti-HA–immunostained U-33 cells documenting constitutively active BMP type 1A receptor (ca-ALK-3) expression; the left panel (Vector) is a negative control. (
d) Effect of increasing concentrations (0–100 ng/ml for 3 days) of PEG-noggin in vector or ca-ALK-3–overexpressing U-33 cells as assessed by ALP activity (given as mean ± SEM), expressed as absolute values. *
P < 0.05, significantly different from untreated control (Vector) cells;
#P < 0.05, significantly different from ca-ALK-3 at 0 concentration, by ANOVA.
