Impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis in noggin-overexpressing mice
J. Clin. Invest. Xue-Bin Wu, et al. 112:924 doi:10.1172/JCI15543 [Go to this article.]

Figure 4
Time course of noggin expression and cell differentiation in calvarial osteoblasts. Calvarial osteoblasts from neonatal noggin^+/– mice were cultured for up to 6 days in the presence of various osteotropic factors. (a) Isolated osteoblasts were cultured for 3 and 6 days in the presence or absence of BMP-2. (b) Time course of β-gal–positive cell numbers in the absence or presence of BMP-2 and TGF-β1. (c) β-gal and ALP activities were measured after 3 days of culture. For these experiments, 300 ng/ml BMP-2, -4, and -6, 5 ng/ml TGF-β1, 200 ng/ml IGF-1, 10^–8 M 1-34 human parathyroid hormone (PTH), 300 ng/ml noggin, and 10^–8 M 1,25(OH)₂D₃ (1,25D₃) were applied. *P < 0.05, significantly different from 0 timepoint (b) or control group (c).