Impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis in noggin-overexpressing mice
J. Clin. Invest. Xue-Bin Wu, et al. 112:924 doi:10.1172/JCI15543 [
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Figure 3Noggin expression in bone marrow–derived macrophages. Bone marrow–derived macrophages (Mφ) isolated from bone marrow cell cultures in the presence of M-CSF examined for noggin and BMP-4 expression by real-time PCR (
a) and Western blot (
b and
c). Expression levels in macrophages in
a were described as a ratio to those in U-33 preosteoblasts. In
b, noggin expression in macrophages and U-33 cells were detected by Western blotting. In
c, macrophages were cultured in the presence or absence of 300 ng/ml BMP-2 for 3 days, and noggin expression was analyzed by Western blotting. *
P < 0.05, significantly different from U-33 cells by ANOVA. Cont, control.
