Renal protection from ischemia mediated by A_2A adenosine receptors on bone marrow–derived cells
J. Clin. Invest. Yuan-Ji Day, et al. 112:883 doi:10.1172/JCI15483 [Go to this article.]

Figure 4
Cytokine and chemokine gene expression from kidneys of chimeric mice subjected to IRI. Kidneys were subjected to 32 minutes of ischemia and either 8 or 24 hours of reperfusion in animals that were treated with either vehicle or ATL146e. V, vehicle; A, ATL146e. (a) RNase protection assay (RPA) analysis of selected cytokines from kidney mRNA using mCK3 probes. Sham-operated chimeric mice (lanes 2 and 7) and chimeric mice subjected to IRI (lanes 3–6 and 8–11). WT→WT mice at 8 (lanes 3 and 4) or 24 (lanes 5 and 6) hours and A_2A-KO→WT mice at 8 (lanes 8 and 9) or 24 (lanes 10 and 11) hours as indicated. Each lane represents solution hybridization with RNA derived from kidneys of separate mice. (b) RPA analysis using the mCK1 probes. (c) RPA analysis using mCK2b probes. WT→WT mice at 24 hours (lanes 1–5) and A_2A-KO→WT mice at 24 hours (lanes 6–8). Mice were subjected to sham operation (S) (lanes 1 and 6) or IRI (lanes 2–5, 7, and 8). Relative mobility of unprotected cytokine mRNA is illustrated on the right. S, sham operation. (d) RPA analysis using mCK5 probes. Kidney RNA was isolated from WT→WT mice at 24 hours (lanes 1–3) and from A_2A-KO→WT mice at 24 hours (lanes 4–6). Mice were subjected to sham operation (lanes 1 and 4) or IRI (lanes 2, 3, 5, and 6).