Renal protection from ischemia mediated by A_2A adenosine receptors on bone marrow–derived cells
J. Clin. Invest. Yuan-Ji Day, et al. 112:883 doi:10.1172/JCI15483 [Go to this article.]

Figure 2
Effect of renal IRI and ATL146e on plasma creatinine in chimeric mice. (a) Reconstitution efficacy of granulocytes and lymphocytes at 6 weeks after BM transplantation. PBMCs from transplanted recipients were purified and incubated with anti-CD45.1 as a marker of donor cells and either anti-CD11b, anti-CD4, or anti-CD8a monoclonal antibodies (see Methods). Labeled cells were enumerated by flow cytometry. The number at the top of each panel indicates the percentage of cells that are positive for both CD45.1 and each of the other marker fluorescent antibodies. Data are representative of 12 independent experiments. (b) Effect of renal IRI and ATL146e on plasma creatinine in chimeric mice. Chimeric mice were prepared by transferring to lethally irradiated WT recipients BM from either WT (WT→WT) or A_2A-KO (A_2A-KO→WT) donor animals. Chimeric animals were treated with vehicle or ATL146e (10 ng/kg per minute) beginning 5 hours before 32 minutes of ischemia and continuing for 24 hours of reperfusion. N = 11 for each group. Values are means ± SE. *P < 0.0001 versus vehicle.