The role of endothelial insulin signaling in the regulation of vascular tone and insulin resistance
J. Clin. Invest. David Vicent, et al. 111:1373 doi:10.1172/JCI15211 [Go to this article.]

Figure 2
Endothelial mediator gene expression in control and VENIRKO endothelial cells. (a) Northern blot analysis of eNOS in heart and aorta. Total RNA (20 μg) from heart and aorta of control and VENIRKO mice (n = 6 each) was fractionated and hybridized to cDNA probes for eNOS and 36B4 mRNA. (b) Real-time quantitative RT-PCR analysis to examine the VEGF, eNOS, and ET-1 in RNA extracts from primary cultures. Two independent sets of endothelial cells were prepared from control and two from VENIRKO mice. Each RNA sample was analyzed by real-time quantitative RT-PCR on three independent experiments. *P < 0.05 for differences between control and VENIRKO mice.