The role of endothelial insulin signaling in the regulation of vascular tone and insulin resistance
J. Clin. Invest. David Vicent, et al. 111:1373 doi:10.1172/JCI15211 [Go to this article.]

Figure 1
Recombination of the IR gene and expression of the IR mRNA in endothelial cells of VENIRKO mice. (a) Schematic representation of the IR/lox allele showing the position of the primers and the recombined IR knockout allele indicating the deletion of exon 4. (b) The PCR product from control mice corresponds to the 266 bp from the amplification between primers 1 and 2 of the endogenous IR gene allele. The PCR product from VENIRKO mice corresponds to the 220 bp from the amplification between primers 2 and 3. (c) Real-time quantitative RT-PCR analysis to determine the IR expression in RNA extracts from primary cultures of control and VENIRKO mice. The expression level of IR in VENIRKO endothelial cells (lane 3 and 4) were reduced to 3–5% of control (lane 1 and 2). *P < 0.01 for differences between control and VENIRKO mice.