Normal Th1 development following long-term therapeutic blockade of CD154-CD40 in experimental autoimmune encephalomyelitis
J. Clin. Invest. Laurence M. Howard, et al. 109:233 doi:10.1172/JCI14374 [
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Figure 6Peripheral T cells from asymptomatic anti-CD154–treated mice demonstrate full encephalitogenic capacity. Splenic (Spl) T cells were isolated from anti-CD154– and control Ig–treated mice 30 days after PLP139-151/CFA immunization. These cells (5 × 10
6 per recipient) were then transferred to naive recipient mice with or without cotransfer of 2 × 10
6 encephalitogenic Th1 blasts. The Th1 blasts were obtained from the draining lymph nodes of mice 10 days after PLP139-151/CFA immunization and 4 days of in vitro reactivation in the presence of PLP139-151 peptide. Recipient mice were observed for clinical signs of disease for 31 days after transfer. Data are representative of two separate experiments with identical results.
