Normal Th1 development following long-term therapeutic blockade of CD154-CD40 in experimental autoimmune encephalomyelitis
J. Clin. Invest. Laurence M. Howard, et al. 109:233 doi:10.1172/JCI14374 [Go to this article.]

Figure 3
Short-term anti-CD154 therapy has no long-term effect on specific proliferative responses. (a) Splenic and lymph node (LN) cells were obtained 100 days after immunization, and in vitro PLP139-151–specific proliferative responses were determined by ³H-TdR incorporation. Data are representative of two separate experiments. (b) Supernatants from proliferative cultures described above were analyzed for secretion of IL-2, IL-4, IL-5, and IFN-γ by capture ELISA. IFN-γ secretion from splenic T cells was significantly reduced in anti-CD154–treated mice. Data are representative of two separate experiments with three mice per experimental group. There was no detectable IL-4 or IL-5 secretion observed from any culture supernatants.