Altered vascular permeability and early onset of experimental autoimmune encephalomyelitis in PECAM-1–deficient mice
J. Clin. Invest. Donnasue Graesser, et al. 109:383 doi:10.1172/JCI13595 [Go to this article.]

Figure 4
Immortalized endothelial cells derived from PECAM-KO mice support increased transmigration of MOG-specific T cells. (a and b) FACS analysis of immortalized endothelial cells derived from WT and KO brain (a) and KO lung and PECAM-reconstituted (RC) lung microvascular endothelial cells (b), illustrating the expression of VE-cadherin and ICAM-1 in all four cell types, but PECAM-1 expression only in the WT and PECAM-RC cells. (c and e) In vitro adhesion assays illustrating no differences in MOG-specific T cell adhesion to WT, PECAM-KO, and PECAM-RC endothelioma cells. (d and f) In vitro transmigration assays similar to those in Figure 2c were performed using MOG-specific T cells transmigrating across monolayers of endothelioma cell lines. Transmigration of T cells across KO endotheliomas derived from brain and lung was consistently and significantly increased in comparison with transmigration across WT and PECAM-RC endotheliomas. In c and d WT T lymphocytes were used, and in e and f KO T lymphocytes were used. n = 4.