Presented antigen from damaged pancreatic β cells activates autoreactive T cells in virus-mediated autoimmune diabetes
J. Clin. Invest. Marc S. Horwitz, et al. 109:79 doi:10.1172/JCI11198 [
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Figure 4Presentation of endogenous antigen by APCs from STZ-treated mice activates β cell–specific BDC T cells. Adherent mononuclear cells from NOD mice treated with STZ or PBS were prepared, irradiated, and cultured with BDC2.5 Tg splenocytes (2 × 10
5) at various ratios. Shown is a summary of results from the 4:1 ratio, T cell (2 × 10
5): APC (5 × 10
4). Also shown are the results of culturing these cells at 4:1 in the presence of antigenic peptide (7 μM) (
20) specific for the BDC T cell. Cultures were pulsed with 1 μCi of
3H thymidine at 48 hours and harvested 18 hours thereafter. The data presented are from four separate experiments involving a total of eight mice in each group representing the APC populations. Values are presented as stimulation index + SEM as compared with BDC splenocytes incubated without additional APCs or antigen. Background in all experiments was between 500 and 2500 cpm.
